{"id":521,"date":"2020-05-02T08:53:23","date_gmt":"2020-05-02T15:53:23","guid":{"rendered":"https:\/\/sites.evergreen.edu\/l3notes\/?p=521"},"modified":"2020-05-02T08:53:24","modified_gmt":"2020-05-02T15:53:24","slug":"dna-extractions","status":"publish","type":"post","link":"https:\/\/sites.evergreen.edu\/l3notes\/dna-extractions\/","title":{"rendered":"DNA Extractions!"},"content":{"rendered":"\n<div class=\"wp-block-image\"><figure class=\"alignright size-thumbnail\"><img loading=\"lazy\" width=\"150\" height=\"150\" src=\"https:\/\/sites.evergreen.edu\/l3notes\/wp-content\/uploads\/sites\/528\/2020\/05\/DNA1-150x150.png\" alt=\"\" class=\"wp-image-524\" \/><figcaption> Litterbag showing willow\u00a0leaf litter\u00a0and\u00a0a unique metal tag for\u00a0identification!\u00a0\u00a0\u00a0 <\/figcaption><\/figure><\/div>\n\n\n\n<p>Across the Pumice Plain, willow leaf litter bags are placed into streams to conduct a variety of assays. After the leaves are colonized by bacteria and fungi and begin to decompose, we remove the leaves and use DNA extractions to identify microbial communities!<\/p>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"alignright size-large\"><img loading=\"lazy\" width=\"901\" height=\"1024\" src=\"https:\/\/sites.evergreen.edu\/l3notes\/wp-content\/uploads\/sites\/528\/2020\/05\/DNA2-901x1024.png\" alt=\"\" class=\"wp-image-523\" srcset=\"https:\/\/sites.evergreen.edu\/l3notes\/wp-content\/uploads\/sites\/528\/2020\/05\/DNA2-901x1024.png 901w, https:\/\/sites.evergreen.edu\/l3notes\/wp-content\/uploads\/sites\/528\/2020\/05\/DNA2-264x300.png 264w, https:\/\/sites.evergreen.edu\/l3notes\/wp-content\/uploads\/sites\/528\/2020\/05\/DNA2-768x873.png 768w, https:\/\/sites.evergreen.edu\/l3notes\/wp-content\/uploads\/sites\/528\/2020\/05\/DNA2-676x768.png 676w, https:\/\/sites.evergreen.edu\/l3notes\/wp-content\/uploads\/sites\/528\/2020\/05\/DNA2.png 921w\" sizes=\"(max-width: 901px) 100vw, 901px\" \/><figcaption>Various stages in the DNA extraction process: Macerated leaf material, intermediate stages of DNA extraction, and final extracts for analysis. Photos by Lauren Thompson <\/figcaption><\/figure><\/div>\n\n\n\n<p>The DNA extraction process entails numerous lengthy, yet exciting steps. Whether its shaking samples up vigorously with a vortexer or lysing cell matter with solutions, Evergreen Undergraduates Lauren and Maddie Thompson are always up for the challenge! <\/p>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-image\"><figure class=\"alignright size-large\"><img loading=\"lazy\" width=\"333\" height=\"276\" src=\"https:\/\/sites.evergreen.edu\/l3notes\/wp-content\/uploads\/sites\/528\/2020\/05\/dna3.png\" alt=\"\" class=\"wp-image-525\" srcset=\"https:\/\/sites.evergreen.edu\/l3notes\/wp-content\/uploads\/sites\/528\/2020\/05\/dna3.png 333w, https:\/\/sites.evergreen.edu\/l3notes\/wp-content\/uploads\/sites\/528\/2020\/05\/dna3-300x249.png 300w\" sizes=\"(max-width: 333px) 100vw, 333px\" \/><figcaption>Undergraduate Maddie Thompson testing for DNA concentrations on the nanodrop! Photo by Lauren Thompson <\/figcaption><\/figure><\/div>\n\n\n\n<p>Extracted DNA from willow samples is then sent off to characterize microbes present! This identifies what microbes colonize first, starts decomposition, provides nutrients to aquatic macroinvertebrates, and may be influenced by the sex of willows!<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Across the Pumice Plain, willow leaf litter bags are placed into streams to conduct a variety of assays. After the leaves are colonized by bacteria and fungi and begin to decompose, we remove the leaves and use DNA extractions to identify microbial communities! The DNA extraction process entails numerous lengthy, yet exciting steps. Whether its [&hellip;]<\/p>\n","protected":false},"author":7504,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_mi_skip_tracking":false},"categories":[6,4,5],"tags":[28,47,58,40,31,44,12,29,26],"_links":{"self":[{"href":"https:\/\/sites.evergreen.edu\/l3notes\/wp-json\/wp\/v2\/posts\/521"}],"collection":[{"href":"https:\/\/sites.evergreen.edu\/l3notes\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.evergreen.edu\/l3notes\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.evergreen.edu\/l3notes\/wp-json\/wp\/v2\/users\/7504"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.evergreen.edu\/l3notes\/wp-json\/wp\/v2\/comments?post=521"}],"version-history":[{"count":0,"href":"https:\/\/sites.evergreen.edu\/l3notes\/wp-json\/wp\/v2\/posts\/521\/revisions"}],"wp:attachment":[{"href":"https:\/\/sites.evergreen.edu\/l3notes\/wp-json\/wp\/v2\/media?parent=521"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.evergreen.edu\/l3notes\/wp-json\/wp\/v2\/categories?post=521"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.evergreen.edu\/l3notes\/wp-json\/wp\/v2\/tags?post=521"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}